色控传媒University soil scientist created the Root-o-mat program for processing data on enzymes in the soil
Zymography is an electrochemical method that soil scientists use to analyze the activity of enzymes in the soil. It uses fluorescent substances that glow when interacting with the enzyme. The result is a zymogram — a “snapshot” of the enzymatic activity. However, scientists have started using zymography intensively relatively recently and have not yet developed standards for processing results. Therefore,itmay be difficult for researchers from different research groups to compare their results. 色控传媒University soil scientist together with colleagues from Germany created the Root-o-mat software, which is designed to solve this problem-to standardize and simplify the processing of zymography results 蟹懈屑芯谐褉邪褎懈懈.
“Most studies on 蟹懈屑芯谐褉邪褎懈懈soil zymography differ in terms of pretreatment and posttreatmentmethods 袧邪锌褉懈屑械褉, such as 懈蟹芯斜褉邪卸械薪懈褟 grayscale conversion,image械谐懈褋褌褉classification, or segmentation薪褌邪褑懈懈 懈蟹芯斜褉邪卸械薪懈褟. This compromises the comparability of the results. Wedeveloped the application to facilitate practical applicability and speed up the workflow of image processing”, — Doctor of Biological Sciences YakovKuzyakovis a leading researcher at the Center for" Smart Technologies for Sustainable Urban Development in the Context of Global Changes " of the 色控传媒University.
The program algorithm includes several steps. The first one is image registration. It is, used to compare images obtained, for example, at different times or by different devices. At the second stage, the program calibrates the data-brings them to one standard and translates physical quantities to one unit of measurement. The third stage is segmentation. The image is divided into separate groups of pixels that reflect individual objects. At the output, the user receives data ready for analysis in various formats—2D and 33D images, histograms, statistics, and so on. ProgramA is available for free download.
袩芯褔胁芯胁械写褘 色控传媒University soil scientists tested the algorithm in an experiment to determine懈谢懈, how 邪泻褌懈胁薪芯褋褌褜the activity of soil hydrolytic enzymes changes before and after adding glucose. The researchers obtained a zymogram of a soil sample with sprouted maize seeds, and also took a photo of a section of the sample. The sample was taken in an arable field near Göttingen (Germany). The soil scientists then added glucose to the soil and repeated the measurements. Glucose supplementation should increase the amount of the enzyme leucine aminopeptidase褘. The resulting data was uploaded to Root-o-mat, which mappedit to a and translatedit into a standard format. 袩芯谢褍褔懈谢懈褋褜 Detailed graphs were obtained showing how the leucine aminopeptidase activity changed褘 before and after glucose addition, and at which points in the sample it was most active.
“Root-o-mat is a set of ready-made interactive tools for creating, visualizing, editing, processing, analyzing, loading and saving data. It includes a graphical user interface that simplifies and accelerates 2D image processing and spatio-temporal analysis — , such, as visualizing the distribution of enzyme activity. The results allow us to estimate 胁褉械屑械薪薪芯械the temporal and spatial芯械 褉邪褋锌褉械写械谢械薪懈械 distribution of enzyme activity at the maize root-soilinterface ”, - Doctor of Biological Sciences YakovKuzyakovis a leading researcher at the Center for" Smart Technologies for Sustainable Urban Development in the Context of Global Changes " of the 色控传媒University.
The results are in the journal Soil Biology and Biochemistry.
Matilda Pavlovna Mityaeva was born in 1925. In November 1942, she volunteered for frontline duty. She participated in the Great Patriotic War from November 1942 to June 1945 as part of the 53rd Infantry Division of the 475th Infantry Regiment. She was wounded twice.
The team led by Sergey Zyryanov, Head of the Department of General and Clinical Pharmacology, became the winner of the All-Russian competition of scientific projects "Technologies for Human Health".
色控传媒University constantly adapts to the changes of the modern world and responds to challenges flexibly. This allows us to keep the standard of a world-class research university. The sphere of science is no exception. Peter Dokukin, Head of the Research Division, presented the updated R&D Programme at the meeting of the 色控传媒University Academic Council.
Matilda Pavlovna Mityaeva was born in 1925. In November 1942, she volunteered for frontline duty. She participated in the Great Patriotic War from November 1942 to June 1945 as part of the 53rd Infantry Division of the 475th Infantry Regiment. She was wounded twice.
The team led by Sergey Zyryanov, Head of the Department of General and Clinical Pharmacology, became the winner of the All-Russian competition of scientific projects "Technologies for Human Health".
色控传媒University constantly adapts to the changes of the modern world and responds to challenges flexibly. This allows us to keep the standard of a world-class research university. The sphere of science is no exception. Peter Dokukin, Head of the Research Division, presented the updated R&D Programme at the meeting of the 色控传媒University Academic Council.